Yeast artificial chromosome (YAC) | What are the components of yeast artificial chromosome?

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  • ℹ️ Published 3 years ago
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this video describes the features and use of YAC as a cloning vector.
Yeast artificial chromosome (YAC) is one engineered vector used to clone DNA sequences in yeast cells. YACs are often used in connection with the mapping and sequencing of genomes. Segments of an organism's DNA, up to one million base pairs in length, can be inserted into YACs. The YACs, with their inserted DNA, are then taken up by yeast cells. As the yeast cells grow and divide, they amplify the YAC DNA, which can be isolated and used for DNA mapping and sequencing.

💬 Comments
Author

As per Gene Cloning & DNA analysis by
T. A brown 6E(P111), the presence of the insert DNA in the vector can be checked by testing for insertional inactivation of SUP4, which is carried out by simple color test, white colonies are recombinants & RED are not recombinants.
While as per Analysis of Genes & Genomes by Richard J Reece: YAC in chapter Artificial chromosomes RED colonies are recombinants.
Now what's correct

Author — Mukaram Bhat

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You have really worked hard for the content of the video and it really worked. Very presentable and interesting images, animations....Keep it up👍👍🙏👏

Author — prathna

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sir its awesome..u have explained in the most practical terms ..these animations will definitely help us retain information in better way..

Author — Megha Patil

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Very good video. You don't miss a point to explain. Like also the positive and negative sites. Really helped me with exam preparation in gene technology. Go on like that :) really cool

Author — Andrea H.

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Well explained in a very short period of time thanks☺

Author — SWATI srivastva

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Thank you Dr
But is there a video explaining the principle of selection of recombinant clones specifically in pYac 2 vector?

Author — Mariam Khatib

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this video helped me more in my research, really thank you so much Dr.

Author — Doaa Yasser

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It is indeed great video..made it so easy to understand!!!.. appreciate your work..thankyou

Author — Sayani Das

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thank you sir, this section was very informative and easy to understand .

Author — Chandini C

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Thank you sooo much! Your videos are really useful and I love the way you explain! And... actually english is not my native language (I'm from Colombia) and honestly, I find it easier to understand people with an accent so... good for me!! haha 
Anyway...keep going with this! please!!

Author — Laura Riascos

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That’s a great job, and it would be greater if you add subtitles.

Author — Aseel Ali

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Helpful video sir..thanku somuch...make more videos like this please

Author — Shafiya Hasan

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One of best Many people had made very lengthy lactures but their content is not Worthwhile ... Best job... And best content...

Author — aubaid shams

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Thanks sir it very help ful and nice visualization ✨️

Author — Ammu Ponnu

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Very helpful sir. I have a presentation this morning on vectors

Author — James Aondoyila

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All videos are great and the speaking is good, understandable. Thanks

Author — Mama

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Thank you so much...This was really helpful to me....

Author — THE SUPER BIOLOGY

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Why do we have to digest vector with BamHI to make it linear? Can't it stay circular?

Author — Magdalena Arendt

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You have a mistake in this video
Recombinant colonies are white in colour and u have mentioned that they are red in colour.

Author — Mr. Tech Freak

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Can you make these videos a little slower and also add a screen when you tell advantages and disadvantages like hearing those details quickly is difficult, if they are written it would be very helpful, And Amazing effort far better than any other explanation videos

Author — gautam moon